Low-cost internal controls for detection of Giardia cysts in water samples
نویسندگان
چکیده
منابع مشابه
Magnetic Separation of Giardia Cysts from Water
Background: Giardia is one of the infectious agents that can be transmitted by surface water and cause a disease. We designed and fabricated a magnetic system to separate the giardia cysts from water. Materials and Methods: In this study, giardia cysts were concentrated by sucrose floatation method. The cysts were mixed with physiological saline and their concentration was adjusted to 100,000 c...
متن کاملEvaluation of immunomagnetic separation and the sucrose flotation methods coupled with immunofluorescence or PCR for detection of Cryptosporidium and Giardia (oo)cysts in water samples
Introduction: Detection of Cryptosporidium and Giardia parasites in water samples is usually performed by US Environmental Protection Agency 1623 method. Nevertheless, the USEPA1623 method still need improvement, to prevent and control the water borne parasitic disease. Therefore, we undertook the present study. Materials and methods: Totally 48 surface water samples were collected. Four sampl...
متن کاملDetection of Giardia cysts with a cDNA probe and applications to water samples.
Giardiasis is the most common human parasite infection in the United States, causing a lengthy course of diarrhea. Transmission of Giardia species is by the fecal-oral route, and numerous waterborne outbreaks have been documented. The Environmental Protection Agency has regulated Giardia species in drinking water through the Surface Water Treatment Rule. Current methods for detection of Giardia...
متن کاملDetection of Giardia lamblia Cysts in Surface Waters of Rasht City, Iran
Introduction: Giardia lamblia is a protozoan parasite with universal distribution in human populations. This infections transfer to human via contaminated foods and waters with Giardia cysts. The Knowledge on the incidence of this agent in the potential infection sources can provide valuable information for control and the spread of this parasite to human communities. This study was aimed to is...
متن کاملAntibody-magnetite method for selective concentration of Giardia lamblia cysts from water samples.
An antibody-magnetite method was developed in order to selectively concentrate Giardia cysts from water samples. The indirect technique employed a mouse immunoglobulin G anti-Giardia antibody as a primary antibody and an anti-mouse immunoglobulin G antibody-coated magnetite particle as a secondary labeling reagent. The magnetically labeled cysts were then concentrated by high-gradient magnetic ...
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ژورنال
عنوان ژورنال: Parasitology International
سال: 2019
ISSN: 1383-5769
DOI: 10.1016/j.parint.2019.04.012